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There are 3 different ways to trigger a conversation with Aiko, slash command, reply to Aiko, and mention Aiko.

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Slash Command

Always work, one of the easiest way to chat with aiko, simply use
Then, Aiko will reply to your words. This method is not affected by server blacklist/whitelist. When quota exhausted, Aiko will reply with an ephemeral reminder.
If slash command is not working, you may want to check “Use Application Commands” permission in that channel, it should be ON.

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Reply to Aiko

Direct way to talk, Aiko traces back up the Discord reply chain for up to five messages and sends them to the language model as conversation history. When quota exhausted, Aiko will reply with a public reminder.

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Mention Aiko

Mention Aiko directly can also trigger conversation, same as slash command, this way do not bring any chat history. When quota exhausted, Aiko will reply with a public reminder.
Server administrators can turn mention and reply responses off, or restrict them to selected channels. This does not disable /aiko.